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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CARS2

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别名:Probable cysteine–tRNA ligase, mitochondrial, Cysteinyl-tRNA synthetase, CysRS, CARS2
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
CARS is a class 2 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid.
It catalyzes the chemical reaction:

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Specification
Aliases Probable cysteine–tRNA ligase, mitochondrial, Cysteinyl-tRNA synthetase, CysRS, CARS2
Entrez GeneID 79587
Swissprot Q9HA77
WB Predicted band size 62.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 536-564 amino acids from the C-terminal region of human CARS2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500

Anti-CARS2 Antibody (C-term) at 1:1000 dilution + NCI-H460 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 62 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CARS2 antibody (C-term) (Cat.#P33667), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。