Rabbit Polyclonal Antibody to CCT3

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别名:T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

Description

CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.

Specification
Aliases	T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, hTRiC5, CCT3, CCTG, TRIC5
Entrez GeneID	7203
Swissprot	P49368
WB Predicted band size	60.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the Central region of human CCT3.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of CCT3 Antibody (Center) (Cat. #P30393) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.
	Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) (Cat. #P30393). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .
	Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.