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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CCT8L2 (N-Term)

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别名:Putative T-complex protein 1 subunit theta-like 2, CCT8L2, CESK1
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
Possible molecular chaperone; assists the folding of proteins upon ATP hydrolysis.
Specification
Aliases Putative T-complex protein 1 subunit theta-like 2, CCT8L2, CESK1
Entrez GeneID 150160
Swissprot Q96SF2
WB Predicted band size 59.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CCT8L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-124 amino acids from human CCT8L2.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-CCT8L2 Antibody (N-Term) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: CCRF-CEM whole cell lysate
Lane 3: A673 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained with P33831 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33831, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。