中文 
别名:T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. |
| Specification | |
|---|---|
| Aliases | T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ |
| Entrez GeneID | 919 |
| Swissprot | P20963 |
| WB Predicted band size | 18.7kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-141 amino acids from the C-terminal region of human CD3Z. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/4000-1/8000 |
| FCM | 1/25 |
 |
All lanes : Anti-CD3Z Antibody (C-term) at 1:4000-1:8000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Mouse thymus lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing K562 cells stained with P34520(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34520, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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