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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CDC25A (S124)

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别名:M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Specification
Aliases M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A
Entrez GeneID 993
Swissprot P30304
WB Predicted band size 59.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
Application
WB 1/1000
IHC 1/100

All lanes : Anti-CDC25A(S124) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of P34629 on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of P34629 on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。