别名:M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl
| Description |
|---|
| Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
| Specification | |
|---|---|
| Aliases | M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A |
| Entrez GeneID | 993 |
| Swissprot | P30304 |
| WB Predicted band size | 59.1kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100 |
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All lanes : Anti-CDC25A(S124) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunohistochemical analysis of P34629 on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
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Immunohistochemical analysis of P34629 on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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