别名:P42; KKIALRE应用:WB,IHC
反应种属:Human
规格:50μl/100μl
Description |
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The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated by the MAP kinases ERK 1 and ERK 2. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. The protein kinase p56 KKIAMRE is distantly related to the MAP kinase group of proteins and is closely related to p42 KKIALRE. KKIAMRE is predominantly expressed in testis, kidney, brain and lung. KKIAMRE contains the conserved MAP kinase dual phosphorylation motif in the sequence Thr-Asp-Tyr and is activated by treatment of cells by EGF. However, unlike other MAP kinases, the EGF-stimulated kinase activity does not require phosphorylation of KKIAMRE and KKIALRE in the Thr-Asp-Tyr motif. |
Specification | |
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Aliases | P42; KKIALRE |
Swissprot | Q00532 |
WB Predicted band size | 42 kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthetic peptide of human CDKL1 |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application | |
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WB | 1/200-1/1000 |
IHC | 1/20-1/100 |
ELISA | 1/5000-1/10000 |
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human prostate tissue and Human stomach tissue lysates, Primary antibody: P06164(CDKL1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute | |
The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P06164(CDKL1 Antibody) at dilution 1/20. (Original magnification: ×200) | |
The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P06164(CDKL1 Antibody) at dilution 1/20. (Original magnification: ×200) |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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