Rabbit Polyclonal Antibody to CDKN1A

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Specification
Aliases	P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Swissprot	P38936
WB Predicted band size	18 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Fusion protein of human CDKN1A
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/40-1/200
ELISA	1/5000-1/10000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: HUVEC cell lysate, Primary antibody: P07186(CDKN1A Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
	The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P07186(CDKN1A Antibody) at dilution 1/45. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P07186(CDKN1A Antibody) at dilution 1/45. (Original magnification: ×200)