Rabbit Polyclonal Antibody to CDKN2B

包装: 1G|100G|500G|1KG|100KG

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别名:Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

Description

This gene lies adjacent to the tumor suppressor gene
CDKN2A in a region that is frequently mutated and deleted in a wide
variety of tumors. This gene encodes a cyclin-dependent kinase
inhibitor, which forms a complex with CDK4 or CDK6, and prevents
the activation of the CDK kinases, thus the encoded protein
functions as a cell growth regulator that controls cell cycle G1
progression. The expression of this gene was found to be
dramatically induced by TGF beta, which suggested its role in the
TGF beta induced growth inhibition. Two alternatively spliced
transcript variants of this gene, which encode distinct proteins,
have been reported.

Specification
Aliases	Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, MTS-2, p14-INK4b, p15-INK4b, p15INK4B, CDKN2B, MTS2
Entrez GeneID	1030
Swissprot	P42772
WB Predicted band size	14.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This CDKN2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the C-terminal region of human CDKN2B.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	Western blot analysis of CDKN2B (arrow) using rabbit polyclonal CDKN2B Antibody (C-term) (Cat. #P31121). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDKN2B gene.
	Confocal immunofluorescent analysis of CDKN2B Antibody (C-term)(Cat#P31121) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	CDKN2B Antibody (C-term) (Cat. #P31121) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	CDKN2B Antibody (C-term) (Cat. #P31121)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN2B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.