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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CE164 (N-term)

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别名:Centrosomal protein of 164 kDa, Cep164, CEP164, KIAA1052, NPHP15
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA,CHK2 and CHK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHK1.
Specification
Aliases Centrosomal protein of 164 kDa, Cep164, CEP164, KIAA1052, NPHP15
Entrez GeneID 22897
Swissprot Q9UPV0
WB Predicted band size 164.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CE164 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-274 amino acids from the N-terminal region of human CE164.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/10-1/50

All lanes : Anti-CE164 Antibody (N-term) at 1:1000 dilution
Lane 1: SH-SY5Y whole cell lysate
Lane 2: A549 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 164 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

CE164 Antibody (N-term) (Cat. #P34248) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。