Rabbit Polyclonal Antibody to CENPE

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别名:KIF10; CENP-E; MCPH13; PPP1R61
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Description

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.

Specification
Aliases	KIF10; CENP-E; MCPH13; PPP1R61
Swissprot	Q02224
WB Predicted band size	316 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human CENPE
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/50-1/100
ELISA	1/5000-1/10000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human vermiform appendix tissue lysate, Primary antibody: P04772(CENPE Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 40 seconds
	The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P04772(CENPE Antibody) at dilution 1/20. (Original magnification: ×200)