中文 
别名:checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; CHFR; E3 ubiquitin-protein ligase CHFR; FLJ10796应用:WB
反应种属:Human,Mouse,Rat
规格：50μl/100μl
| Description |
|---|
| E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the ‘Lys-48’-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of ‘Lys-63’-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at ‘Lys-63’ are usually not targeted for degradation, but are rather involved in signaling cellular stress.Scolnick D.M., Nature 406:430-435(2000).Ota T., Nat. Genet. 36:40-45(2004).Scherer S.E., Nature 440:346-351(2006) |
| Specification | |
|---|---|
| Aliases | checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; CHFR; E3 ubiquitin-protein ligase CHFR; FLJ10796 |
| Entrez GeneID | 55743; |
| Swissprot | Q96EP1 |
| WB Predicted band size | 74kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from Internal of human CHFR. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/3000 |
 |
Western blot analysis of extracts from Jurkat cells, using CHFR antibody P42717. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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