中文 
别名:Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs. |
| Specification | |
|---|---|
| Aliases | Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 |
| Entrez GeneID | 1111 |
| Swissprot | O14757 |
| WB Predicted band size | 54.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-455 amino acids from the C-terminal region of human CHK1. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
 |
All lanes : Anti-CHK1 Antibody (D440) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse colon lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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