Rabbit Polyclonal Antibody to Chk2 (Ab-68)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at ‘Ser-20’ by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.Gorgoulis VG, et al. (2005) Nature; 434(7035): 907-13.Falck J, et al. (2005) Nature; 434(7033): 605-11.Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81.Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.

Description

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at ‘Ser-20’ by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.Gorgoulis VG, et al. (2005) Nature; 434(7035): 907-13.Falck J, et al. (2005) Nature; 434(7033): 605-11.Jin ZH, et al. (2005) Oncogene; 24(12): 1973-81.Li J, et al. (2005) J Biol Chem; 280(12): 12041-50.

Specification
Aliases	CHEK2; CHK2; Cds1; Chk2; EC 2.7.11.1
Entrez GeneID	11200;
Swissprot	O96017
WB Predicted band size	62kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 68.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 (Ab-68) antibody P41688.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 (Ab-68) antibody P41688.