Rabbit Polyclonal Antibody to Chk2 (Phospho-Thr387)

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别名:RAD53; CHK2; Cds1; RAD53;
应用:WB
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.Guo X, et al. (2010)J Biol Chem 285, 33348-57 Carlessi L, Buscemi G, Fontanella E, Delia D (2010)Biochim Biophys Acta 1803, 1213-23 Li J, et al. (2008)J Biol Chem 283, 36019-30

Description

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.Guo X, et al. (2010)J Biol Chem 285, 33348-57 Carlessi L, Buscemi G, Fontanella E, Delia D (2010)Biochim Biophys Acta 1803, 1213-23 Li J, et al. (2008)J Biol Chem 283, 36019-30

Specification
Aliases	RAD53; CHK2; Cds1; RAD53;
Entrez GeneID	11200;
Swissprot	O96017
WB Predicted band size	62kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of threonine 387 (C-G-T(p)-P-T) derived from Human Chk2.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000

Western blot analysis of extracts from Jurkat tissue using Chk2 (Phospho-Thr387) antibody P40573.The lane on the right is treated with the antigen-specific peptide.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。

Rabbit Polyclonal Antibody to Chk2 (Phospho-Thr387)

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