中文 
别名:Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2应用:WB,ICC,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. |
| Specification | |
|---|---|
| Aliases | Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2 |
| Entrez GeneID | 1164 |
| Swissprot | P33552 |
| WB Predicted band size | 9.9kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CKS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 43-77 amino acids from the human region of human CKS2. |
| Application | |
|---|---|
| WB | 1/2000 |
| ICC | 1/25 |
| FCM | 1/25 |
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All lanes : Anti-CKS2 Antibody (C-Term) at 1:2000 dilution Lane 1: Caco-2 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling CKS2 with P34485 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue). |
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Overlay histogram showing A549 cells stained with P34485(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34485, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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