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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CKS2 (N-Term)

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别名:Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2
应用:WB,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Specification
Aliases Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2
Entrez GeneID 1164
Swissprot P33552
WB Predicted band size 9.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This CKS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the human region of human CKS2.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-CKS2 Antibody (N-Term) at 1:2000 dilution
Lane 1: Caco2 whole cell lysate
Lane 2: RPMI 8226 whole cell lysate
Lane 3: SH-SY5Y whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 10 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells stained with P34507(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34507, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。