Rabbit Polyclonal Antibody to CLDN2 (C-term Y224)

包装: 1G|100G|500G|1KG|100KG

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别名:Claudin-2, SP82, CLDN2
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5′ untranslated region have been found for this gene.

Description

This gene product belongs to the claudin protein family
whose members have been identified as major integral membrane
proteins localized exclusively at tight junctions. Claudins are
expressed in an organ-specific manner and regulate tissue-specific
physiologic properties of tight junctions. This protein is
expressed in the intestine. Alternatively spliced transcript
variants with different 5′ untranslated region have been found for
this gene.

Specification
Aliases	Claudin-2, SP82, CLDN2
Entrez GeneID	9075
Swissprot	P57739
WB Predicted band size	24.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Anti-CLDN2 Antibody at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224) (Cat. #P33609). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.
	CLDN2 Antibody (C-term Y224) (Cat. #P33609) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	CLDN2 Antibody (C-term Y224) (Cat. #P33609)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated.