Rabbit Polyclonal Antibody to CLNS1A

包装: 1G|100G|500G|1KG|100KG

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别名:Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, I(Cln), Chloride ion current inducer protein, ClCI, Reticulocyte pICln, CLNS1A, CLCI, ICLN
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
CLNS1A is a protein that functions in multiple regulatory pathways. The protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator.

Specification
Aliases	Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, I(Cln), Chloride ion current inducer protein, ClCI, Reticulocyte pICln, CLNS1A, CLCI, ICLN
Entrez GeneID	1207
Swissprot	P54105
WB Predicted band size	26.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the C-terminal region of human CLNS1A.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000-1/8000
IHC	1/100-1/500
FCM	1/10-1/50

	All lanes : Anti-CLNS1A Antibody (C-term) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Western blot analysis of CLNS1A antibody (C-term) (Cat.# P33856) in HL60 cell line lysates (35ug/lane). CLNS1A (arrow) was detected using the purified Pab.
	Flow cytometric analysis of hela cells using CLNS1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.