Rabbit Polyclonal Antibody to Cofilin

包装: 1G|100G|500G|1KG|100KG

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别名:CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform
应用:WB,IHC,ICC
反应种属:Human,Mouse
规格：50μl/100μl

Description
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Specification
Aliases	CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform
Entrez GeneID	1072
Swissprot	P23528
WB Predicted band size	Calculated MW: 19 kDa; Observed MW: 19 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human coflin1/cofilin2.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Cofilin in MDA lysates using coflin1/cofilin2 (Ab88) antibody.
	Immunofluorescence analysis of Cofilin in Hela using coflin1/cofilin2 (Ab88) antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using coflin1/cofilin2 (Ab88) antibody (left) or the same antibody preincubated with blocking peptide (right).High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.