别名:CFL; CFL1;应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格:50μl/100μl
Specification | |
---|---|
Aliases | CFL; CFL1; |
Entrez GeneID | 1072; |
Swissprot | P23528 |
WB Predicted band size | 19kDa |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin. |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application | |
---|---|
WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
ICC | 1/100-1/200 |
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Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody P40135(Lane 2) and the same antibody preincubated with blocking peptide(Lane1). |
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Lane 1 : HelaΒ treated with H2O2 Lane 2 : Mouse brain tissue Lane 3 : Rat brain tissue Lane 4 : N-peptide blocked HelaΒ treated with H2O2 Lane 5 : N-peptide blocked Mouse brain tissue Lane 6 : N-peptide blocked Rat brain tissue Lane 7 : P-peptide blocked HelaΒ treated with H2O2 Lane 8 : P-peptide blocked Mouse brain tissue Lane 9 : P-peptide blocked Rat brain tissue Lysates/proteins at 40 μg per lane Predicted band size :Β 19 kDa Observed band size :Β 19 kDa |
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Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody P40135. |
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody P40135(left) or the same antibody preincubated with blocking peptide(right). |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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