Rabbit Polyclonal Antibody to COG4

包装: 1G|100G|500G|1KG|100KG

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别名:Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.

Description

Multiprotein complexes are key determinants of Golgi apparatus structure and its capacity for intracellular transport and glycoprotein modification. Several complexes have been identified, including the Golgi transport complex (GTC), the LDLC complex, which is involved in glycosylation reactions, and the SEC34 complex, which is involved in vesicular transport. These 3 complexes are identical and have been termed the conserved oligomeric Golgi (COG) complex, which includes COG4.

Specification
Aliases	Conserved oligomeric Golgi complex subunit 4, COG complex subunit 4, Component of oligomeric Golgi complex 4, COG4
Entrez GeneID	25839
Swissprot	Q9H9E3
WB Predicted band size	89.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This COG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-785 amino acids from the C-terminal region of human COG4.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Anti-COG4 Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Western blot analysis of COG4 Antibody (C-term) (Cat.#P33865) in A2058 cell line lysates (35ug/lane). COG4 (arrow) was detected using the purified Pab.
	Flow cytometric analysis of jurkat cells using COG4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human skeletal muscle with COG4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.