Rabbit Polyclonal Antibody to COL5A1 (N-term)

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别名:Collagen alpha-1(V) chain, COL5A1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [provided by RefSeq].

Description

COL5A1 encodes an alpha chain for one of the low
abundance fibrillar collagens. Fibrillar collagen molecules are
trimers that can be composed of one or more types of alpha chains.
Type V collagen is found in tissues containing type I collagen and
appears to regulate the assembly of heterotypic fibers composed of
both type I and type V collagen. COL5A1 product is closely
related to type XI collagen and it is possible that the collagen
chains of types V and XI constitute a single collagen type with
tissue-specific chain combinations. Mutations in this gene are
associated with Ehlers-Danlos syndrome, types I and II. [provided
by RefSeq].

Specification
Aliases	Collagen alpha-1(V) chain, COL5A1
Entrez GeneID	1289
Swissprot	P20908
WB Predicted band size	183.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000-1/2000
IHC	1/100-1/500

	Anti-COL5A1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	COL5A1 Antibody (N-term) (Cat. #P34604) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).
	Immunohistochemical analysis of paraffin-embedded Human heart tissue using P34604 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded Human uterus tissue using P34604 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	COL5A1 antibody (N-term) (Cat. #P34604) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.