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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to Connexin 36

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别名:Gap junction delta-2 protein, Connexin-36, Cx36, Gap junction alpha-9 protein, GJD2, GJA9
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.
Specification
Aliases Gap junction delta-2 protein, Connexin-36, Cx36, Gap junction alpha-9 protein, GJD2, GJA9
Entrez GeneID 57369
Swissprot Q9UKL4
WB Predicted band size 36.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This Connexin 36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-321 amino acids from the C-terminal region of human Connexin 36.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/2000
IHC 1/100-1/500

All lanes : Anti-Connexin 36 Antibody (C-term) at 1:2000 dilution
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lane 3: rat brain lysate
Lane 4: SH-SY5Y whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 36kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

The anti-GJA9 C-term Pab (Cat. #P33619) is used in Western blot to detect GJA9 in placenta tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。