中文 
别名:Gap junction alpha-5 protein, Connexin-40, Cx40, GJA5应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua. |
| Specification | |
|---|---|
| Aliases | Gap junction alpha-5 protein, Connexin-40, Cx40, GJA5 |
| Entrez GeneID | 2702 |
| Swissprot | P36382 |
| WB Predicted band size | 40.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
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All lanes : Anti-Connexin 40 Antibody (N-term) at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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All lanes : Anti-Connexin 40 Antibody (N-term) at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunohistochemical analysis of paraffin-embedded human stomach tissue using P34578 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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