Rabbit Polyclonal Antibody to CRYBA1

包装: 1G|100G|500G|1KG|100KG

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别名:Beta-crystallin A3, Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form, CRYBA1, CRYB1
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease ‘zonular cataract with sutural opacities’.

Description

Crystallins are separated into two classes:
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Beta-crystallins, the most
heterogeneous, differ by the presence of the C-terminal extension
(present in the basic group, none in the acidic group).
Beta-crystallins form aggregates of different sizes and are able to
self-associate to form dimers or to form heterodimers with other
beta-crystallins. This gene, a beta acidic group member, encodes
two proteins (crystallin, beta A3 and crystallin, beta A1) from a
single mRNA, the latter protein is 17 aa shorter than crystallin,
beta A3 and is generated by use of an alternate translation
initiation site. Deletion of exons 3 and 4 causes the autosomal
dominant disease ‘zonular cataract with sutural opacities’.

Specification
Aliases	Beta-crystallin A3, Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form, CRYBA1, CRYB1
Entrez GeneID	1411
Swissprot	P05813
WB Predicted band size	25.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This CRYBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human CRYBA1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	CRYBA1 Antibody (Center) (Cat. #P31075) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CRYBA1 antibody detected the CRYBA1 protein (arrow).
	CRYBA1 Antibody (Center) (Cat. #P31075) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the CRYBA1 antibody detected the CRYBA1 protein (arrow).
	CRYBA1 Antibody (Center) (Cat. #P31075)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRYBA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.