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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CSNK1G2

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别名:Casein kinase I isoform gamma-2, CKI-gamma 2, CSNK1G2, CK1G2
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The casein kinase 1 (CK1) group consists of 12 kinases including CK1, TTBK (tau tubulin kinase), and VRK (vaccinia-related kinase) families.
The receptor guanylate cyclase (RGC) group consists of 5 kinases similar in domain sequence to TKs (ANP, CYG).
Specification
Aliases Casein kinase I isoform gamma-2, CKI-gamma 2, CSNK1G2, CK1G2
Entrez GeneID 1455
Swissprot P78368
WB Predicted band size 47.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CSNK1G2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-360 amino acids from the C-terminal region of human CSNK1G2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500

Anti-CK1g2 Antibody (D344) at 1:1000 dilution + HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 47 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CK1g2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。