Rabbit Polyclonal Antibody to CTNNB1

包装: 1G|100G|500G|1KG|100KG

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别名:Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
应用:WB,IHC,ICC,FCM
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Description

The protein encoded by this gene is part of a complex of
proteins that constitute adherens junctions (AJs). AJs are
necessary for the creation and maintenance of epithelial cell
layers by regulating cell growth and adhesion between cells. The
encoded protein also anchors the actin cytoskeleton and may be
responsible for transmitting the contact inhibition signal that
causes cells to stop dividing once the epithelial sheet is
complete. Finally, this protein binds to the product of the APC
gene, which is mutated in adenomatous polyposis of the colon.
Mutations in this gene are a cause of colorectal cancer (CRC),
pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.
Three transcript variants encoding the same protein have been found
for this gene.

Specification
Aliases	Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
Entrez GeneID	1499
Swissprot	P35222
WB Predicted band size	85.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This CTNNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 692-721 amino acids from the C-terminal region of human CTNNB1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/2000
IHC	1/100-1/500
ICC	1/50
FCM	1/50

	CTNNB1 Antibody (C-term) (Cat. #P32453) western blot analysis in 293,T47D cell line lysates (35ug/lane).This demonstrates the CTNNB1 antibody detected the CTNNB1 protein (arrow).
	Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	CTNNB1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.
	CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTNNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	CTNNB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTNNB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.