中文 
别名:应用:WB
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. |
| Specification | |
|---|---|
| Swissprot | Q13619 |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CUL4A |
| Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Application | |
|---|---|
| WB | 1/500-1/2000 |
| ELISA | 1/2000-1/5000 |
 |
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Human fetal muscle tissue, Human fetal brain tissue, Hela cells, Primary antibody: P03844(CUL4A Antibody) at dilution 1/325, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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