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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to CX3CL1

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别名:Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, Processed fractalkine, CX3CL1, FKN, NTT, SCYD1
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Specification
Aliases Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, Processed fractalkine, CX3CL1, FKN, NTT, SCYD1
Entrez GeneID 6376
Swissprot P78423
WB Predicted band size 42.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CX3CL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-306 amino acids from the Central region of human CX3CL1.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-CX3CL1 Antibody (Center) at 1:1000-1:2000 dilution
Lane 1: Human brain lysate
Lane 2: Human heart lysate
Lane 3: Human lung lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells stained with P34433(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34433, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。