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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to DDX11

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别名:Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, hCHLR1, DEAD/H box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1, CHLR1, KRG2
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
DEAD box proteins, characterized by the conserved motif
Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
implicated in a number of cellular processes involving alteration
of RNA secondary structure such as translation initiation, nuclear
and mitochondrial splicing, and ribosome and spliceosome assembly.
Based on their distribution patterns, some members of this family
are believed to be involved in embryogenesis, spermatogenesis, and
cellular growth and division. This gene encodes a DEAD box protein,
which is an enzyme that possesses both ATPase and DNA helicase
activities. This gene is a homolog of the yeast CHL1 gene, and may
function to maintain chromosome transmission fidelity and genome
stability. Alternative splicing results in multiple transcript
variants encoding distinct isoforms.
Specification
Aliases Probable ATP-dependent RNA helicase DDX11, CHL1-related protein 1, hCHLR1, DEAD/H box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1, CHLR1, KRG2
Entrez GeneID 1663
Swissprot Q96FC9
WB Predicted band size 108.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 819-847 amino acids from the C-terminal region of human DDX11.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/10-1/50

All lanes : Anti-DDX11 Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 108 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

DDX11 Antibody (C-term) (Cat. #P34086) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。