Rabbit Polyclonal Antibody to DENND1B (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Description

Clathrin (see MIM 118955)-mediated endocytosis is a major
mechanism for internalization of proteins and lipids. Members of
the connecdenn family, such as DENND1B, function as guanine
nucleotide exchange factors (GEFs) for the early endosomal small
GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor
protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35
activation with the clathrin machinery (Marat and McPherson, 2010
[PubMed 20154091]).

Specification
Aliases	DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B, DENND1B, C1orf218, FAM31B
Entrez GeneID	163486
Swissprot	Q6P3S1
WB Predicted band size	86.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/500-1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Anti-DENND1B Antibody (N-term) at 1:500 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	DENND1B Antibody (N-term) (Cat. #P33691) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).
	DENND1B Antibody (N-term) (Cat. #P33691) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	DENND1B antibody (N-term) (Cat. #P33691) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.