Rabbit Polyclonal Antibody to DFFB

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:DNA fragmentation factor subunit beta, 3—, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Specification
Aliases	DNA fragmentation factor subunit beta, 3—, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
Entrez GeneID	1677
Swissprot	O76075
WB Predicted band size	39.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/25

	Western blot analysis of lysate from A431 cell line, using DFFB Antibody (Center)(Cat. #P32643). P32643 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green, Cat#P32643) compared to an isotype control of rabbit IgG(blue). P32643 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center)(Cat#P32643). P32643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
	Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center)(Cat#P32643). P32643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.