Rabbit Polyclonal Antibody to DNAJB1

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别名:Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Description

Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins.

Specification
Aliases	Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Swissprot	P25685
WB Predicted band size	38 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Fusion protein of human DNAJB1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/200
ELISA	1/2000-1/5000

	Gel: 15%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: P07921(DNAJB1 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
	The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P07921(DNAJB1 Antibody) at dilution 1/40. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P07921(DNAJB1 Antibody) at dilution 1/40. (Original magnification: ×200)