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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to DYNC1H1

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别名:Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, DYNC1H1, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Specification
Aliases Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, DYNC1H1, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325
Entrez GeneID 1778
Swissprot Q14204
WB Predicted band size 532.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000-1/2000
IHC 1/100-1/500

All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: U-87 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 532 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: mouse brain lysate
Lane 2: PC-3 whole cell lysate
Lane 3: U-87 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 532 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: mouse brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 532 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P34285 staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。