Rabbit Polyclonal Antibody to EDD

包装: 1G|100G|500G|1KG|100KG

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别名:CAK; CD167a antigen; Cell adhesion kinase; Discoidin receptor tyrosine kinase; EC 2.7.1.112
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of ‘Lys-63’-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.Michelle J. Henderson, J. Biol. Chem., Dec 2006; 281: 39990 – 40000. Lisa N. Kinch, Protein Sci., Feb 2005; 14: 360 – 367. Michelle J. Henderson, J. Biol. Chem., Jul 2002; 277: 26468 – 26478. Darren N. Saunders, Mol. Cell. Biol., Aug 2004; 24: 7225 – 7

Description

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of ‘Lys-63’-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.Michelle J. Henderson, J. Biol. Chem., Dec 2006; 281: 39990 – 40000. Lisa N. Kinch, Protein Sci., Feb 2005; 14: 360 – 367. Michelle J. Henderson, J. Biol. Chem., Jul 2002; 277: 26468 – 26478. Darren N. Saunders, Mol. Cell. Biol., Aug 2004; 24: 7225 – 7

Specification
Aliases	CAK; CD167a antigen; Cell adhesion kinase; Discoidin receptor tyrosine kinase; EC 2.7.1.112
Entrez GeneID	51366;
Swissprot	O95071
WB Predicted band size	309kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from Internal of human EDD.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from A549 cells, using EDD antibody P41839.
	Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using EDD antiobdy P41839. The lane on the left is treated with systhesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EDD antibody P41839.