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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to EEF1B2

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别名:Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
应用:WB,IHC,ICC,FCM
反应种属:Human
规格:50μl/100μl

Description
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
Specification
Aliases Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
Entrez GeneID 1933
Swissprot P24534
WB Predicted band size 24.8kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.
Application
WB 1/1000
IHC 1/100-1/500
ICC 1/25
FCM 1/25

Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody (Center)(Cat. #P32608). P32608 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Fluorescent image of Hela cells stained with EEF1B2 Antibody (Center)(Cat#P32608). P32608 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Flow cytometric analysis of Hela cells using EEF1B2 Antibody (Center)(green, Cat#P32608) compared to an isotype control of rabbit IgG(blue). P32608 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody (Center)(Cat#P32608). P32608 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。