Rabbit Polyclonal Antibody to EEF1D

包装: 1G|100G|500G|1KG|100KG

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别名:EF1D; EF-1D; FP1047
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Specification
Aliases	EF1D; EF-1D; FP1047
Swissprot	P29692
WB Predicted band size	31 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human EEF1D
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/1000-1/5000
IHC	1/50-1/300
ELISA	1/5000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HepG2, A172 and A549 cell lysates, Primary antibody: P03206(EEF1D Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds
	The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P03206(EEF1D Antibody) at dilution 1/55. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P03206(EEF1D Antibody) at dilution 1/55. (Original magnification: ×200)