Rabbit Polyclonal Antibody to EEF1E1

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别名:P18; AIMP3
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.

Description

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.

Specification
Aliases	P18; AIMP3
Swissprot	O43324
WB Predicted band size	20 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Full length fusion protein
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/25-1/100
ELISA	1/5000-1/10000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-6: A549 cell, Jurkat cell, Human testis tissue, 293T cell, Hepg2 cell and A431 cell, Primary antibody: P09146(EEF1E1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds
	The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09146(EEF1E1 Antibody) at dilution 1/35. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P09146(EEF1E1 Antibody) at dilution 1/35. (Original magnification: ×200)