Rabbit Polyclonal Antibody to EFNA1

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Ephrin-A1 [Precursor]; EPH-related receptor tyrosine kinase ligand 1; LERK-1; Immediate early response protein B61; Tumor necrosis factor
应用:WB,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.Holzman L.B., Mol. Cell. Biol. 10:5830-5838(1990).Gregory S.G., Nature 441:315-321(2006).Zhang Z., Protein Sci. 13:2819-2824(2004).

Description

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.Holzman L.B., Mol. Cell. Biol. 10:5830-5838(1990).Gregory S.G., Nature 441:315-321(2006).Zhang Z., Protein Sci. 13:2819-2824(2004).

Specification
Aliases	Ephrin-A1 [Precursor]; EPH-related receptor tyrosine kinase ligand 1; LERK-1; Immediate early response protein B61; Tumor necrosis factor
Entrez GeneID	1942;
Swissprot	P20827
WB Predicted band size	24kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from internal of human EFNA1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from 293 cells and Jurkat cells, using EFNA1 antibody P41922.
	Immunofluorescence analysis of HeLa cells, using EFNA1 antibody P41922.