Rabbit Polyclonal Antibody to EGFR (Ab-1172)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.10.1; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian); Epidermal growth factor receptor precursor; ERBB1
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.Ullrich A., Nature 309:418-425(1984).Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995).Reiter J.L., Nucleic Acids Res. 24:4050-4056(1996)

Description

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.Ullrich A., Nature 309:418-425(1984).Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995).Reiter J.L., Nucleic Acids Res. 24:4050-4056(1996)

Specification
Aliases	EC 2.7.10.1; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian); Epidermal growth factor receptor precursor; ERBB1
Entrez GeneID	1956;
Swissprot	P00533
WB Predicted band size	175kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from HT-29 cells, using EGFR (Ab-1172) antibody P41690.
	Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using EGFR (Ab-1172) antiobdy P41690. The lane on the left is treated with synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EGFR (Ab-1172) antibody P41690.