Rabbit Polyclonal Antibody to eIF2B (Ab-67)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:DKFZp686L18198; eIF-2-beta; EIF2; EIF2B; EIF2beta
应用:WB
反应种属:Human,Mouse
规格：50μl/100μl

Description
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.Pathak V.K., Cell 54:633-639(1988).Deloukas P., Nature 414:865-871(2001).Olsen J.V., Cell 127:635-648(2006).

Description

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.Pathak V.K., Cell 54:633-639(1988).Deloukas P., Nature 414:865-871(2001).Olsen J.V., Cell 127:635-648(2006).

Specification
Aliases	DKFZp686L18198; eIF-2-beta; EIF2; EIF2B; EIF2beta
Entrez GeneID	8894;
Swissprot	P20042
WB Predicted band size	40kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized peptide derived from N-terminal of human eIF2B.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000

	Western blot analysis of extracts from K562 cells, using eIF2B (Ab-67) antibody P41787.
	Western blot analysis of extracts from JK cells (Lane 2), using eIF2B (Ab-67) antiobdy P41787. The lane on the left is treated with systhesized peptide.