Rabbit Polyclonal Antibody to EIF2S1

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Description

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Specification
Aliases	EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Swissprot	P05198
WB Predicted band size	36 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide of human EIF2S1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/30-1/150
ELISA	1/5000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-6: A549 cell, Human placenta tissue, A172 cell, NIH/3T3 cell, Jurkat cell, Hela cell lysates, Primary antibody: P13304(EIF2S1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
	The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P13304(EIF2S1 Antibody) at dilution 1/20. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P13304(EIF2S1 Antibody) at dilution 1/20. (Original magnification: ×200)