Rabbit Polyclonal Antibody to EIF2S1 (N-term)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
应用:WB,IHC,ICC
反应种属:Mouse
规格：50μl/100μl

Description
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Description

The translation initiation factor EIF2 catalyzes the first
regulated step of protein synthesis initiation, promoting the
binding of the initiator tRNA to 40S ribosomal subunits. Binding
occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2
is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha
subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908),
and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of
formation of the ternary complex is modulated by the
phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed
2948954]).

Specification
Aliases	Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Entrez GeneID	1965
Swissprot	P05198
WB Predicted band size	36.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Mouse
Immunogen	This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	EIF2S1 Antibody (N-term) (Cat. #P31291) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF2S1 antibody detected the EIF2S1 protein (arrow).
	Confocal immunofluorescent analysis of EIF2S1 Antibody (N-term) (Cat#P31291) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	EIF2S1 Antibody (N-term) (Cat. #P31291)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.