Rabbit Polyclonal Antibody to EIF4G2

包装: 1G|100G|500G|1KG|100KG

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别名:Eukaryotic translation initiation factor 4 gamma 2, P97, AAG1, DAP5, NAT1
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Description

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Specification
Aliases	Eukaryotic translation initiation factor 4 gamma 2, P97, AAG1, DAP5, NAT1
Entrez GeneID	1982;
Swissprot	P78344
WB Predicted band size	102kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthetic peptide corresponding to a region derived from 796-812 amino acids of Human Eukaryotic translation initiation factor 4 gamma 2
Formulation	Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Application
WB	1/200-1/1000
IHC	110-1/50
ELISA	1:500-1:5000

	Gel: 8%SDS-PAGELane1: Hela cell lyasteLane2: A549 cell lyasteLysates: 40 ug per lanePrimary antibody: 1/400 dilutionSecondary antibody: Donkey anti Rabbit IgG – H&L (HRP) at 1/5000 dilutionExposure time: 40 seconds
	The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31184 (EIF4G2 Antibody) at dilution 1/25, on the right is treated with the synthetic peptide.