Rabbit Polyclonal Antibody to EN1

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别名:
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the ‘engrailed’ (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Description

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the ‘engrailed’ (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Specification
Swissprot	Q05925
WB Predicted band size	40 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human EN1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/20-1/100
ELISA	1/5000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A172 and HEPG2 cell lysates, Primary antibody: P13321(EN1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
	The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P13321(EN1 Antibody) at dilution 1/20. (Original magnification: ×200)