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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ENAM

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别名:Enamelin, ENAM
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Specification
Aliases Enamelin, ENAM
Entrez GeneID 10117
Swissprot Q9NRM1
WB Predicted band size 128.8kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ENAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1097-1126 amino acids from the C-terminal region of human ENAM.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

All lanes : Anti-ENAM Antibody (C-term) at 1:1000 dilution
Lane 1: U-87 MG whole cell lysate
Lane 2: K562 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.

Observed band size : 95kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ENAM Antibody (C-term) at 1:1000 dilution
Lane 1: K562 whole cell lysate
Lane 2: U-87 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.

Observed band size : 95kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

ENAM Antibody (C-term) (Cat. #P34939) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ENAM antibody detected the ENAM protein (arrow).
ENAM Antibody (C-term) (Cat. #P34939) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ENAM antibody(C-term) (Cat. #P34939) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENAM antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。