别名:Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl
Description |
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Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol. |
Specification | |
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Aliases | Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE |
Entrez GeneID | 64772 |
Swissprot | Q8NFI3 |
WB Predicted band size | 84.0kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/2000 |
IHC | 1/100-1/500 |
FCM | 1/10-1/50 |
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All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: human brain tissue lysate Lane 3: mouse thymus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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ENASE Antibody (Center) (Cat. #P34659) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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ENASE Antibody (Center) (Cat. #P34659) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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