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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ENOX2 (N-term)

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别名:Ecto-NOX disulfide-thiol exchanger 2, APK1 antigen, Cytosolic ovarian carcinoma antigen 1, Tumor-associated hydroquinone oxidase, tNOX, Hydroquinone [NADH] oxidase, 1—, Protein disulfide-thiol oxidoreductase, 1—, ENOX2, COVA1
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
The protein encoded by this gene is a growth-related cell
surface protein. It was identifed because it reacts with the
monoclonal antibody KI in cells, such as the ovarian carcinoma line
OVCAR-3, also expressing the CAKI surface glycoprotein. The encoded
protein has two enzymatic activities: catalysis of hydroquinone or
NADH oxidation, and protein disulfide interchange. The two
activities alternate with a period length of about 24 minutes. The
encoded protein also displays prion-like properties. Two transcript
variants encoding different isoforms have been found for this gene.
Specification
Aliases Ecto-NOX disulfide-thiol exchanger 2, APK1 antigen, Cytosolic ovarian carcinoma antigen 1, Tumor-associated hydroquinone oxidase, tNOX, Hydroquinone [NADH] oxidase, 1—, Protein disulfide-thiol oxidoreductase, 1—, ENOX2, COVA1
Entrez GeneID 10495
Swissprot Q16206
WB Predicted band size 70.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ENOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ENOX2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
IHC 1/100

Anti-ENOX2 Antibody (N-term) at 1:2000 dilution + K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 70 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of P34768 on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。