Rabbit Polyclonal Antibody to EphB6 (N-term S45)

包装: 1G|100G|500G|1KG|100KG

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别名:Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Specification
Aliases	Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
Entrez GeneID	2051
Swissprot	O15197
WB Predicted band size	110.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human EphB6.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000-1/2000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of anti-EphB6 N-term Pab (Cat. #P30039) in A549 cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Flow cytometric analysis of MCF-7 cells using EphB6 Antibody (N-term S45) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.