中文 
别名:EFNB2; HTKL; LERK5应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | EFNB2; HTKL; LERK5 |
| Entrez GeneID | 1948; |
| Swissprot | P52799 |
| WB Predicted band size | 37kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2). |
| Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Application | |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/100-1/200 |
| ICC | 1/100-1/200 |
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Gel: 8% SDS-PAGELysate: 40 ugLane: A549 cell lysatePrimary antibody: Ephrin-B2(Ab-330) Antibody at dilution 1/800Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilutionExposure time: 10 seconds |
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All Lanes: Ephrin-B2(Ab-330) Antibody at 1/1000 dilution. |
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded hela cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary. |
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Immunofluorescent analysis of formalin-fixed, paraffin-embedded C6 cells with 21196-lot 7326 at 1/75 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary. |
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21196-lot 7326 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. |
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21196-lot 7326 at 1/200 staining human meningeal carcinomatosis(MC) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary |
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21196-lot 7326 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary |
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21196-lot 7326 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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